MYCOLab Ai Logo - gold and army green

Telomere-to-telomere genome assembly of matsutake (Tricholoma matsutake)

Summary

Scientists have successfully sequenced the complete genome of matsutake mushrooms for the first time. Using advanced DNA sequencing technology, they created a map of all 13 matsutake chromosomes containing about 22,000 genes. This achievement provides crucial information to help understand, preserve, and possibly improve this highly valued but increasingly rare mushroom that has been prized in Japanese cuisine for over a thousand years.

Background

Matsutake is a highly valued ectomycorrhizal fungus used in traditional Japanese cuisine, but its sporocarps are non-culturable and production has drastically decreased. Four previous matsutake genome assemblies were highly fragmented with insufficient coverage. Advanced sequencing technologies now enable complete telomere-to-telomere genome assembly.

Objective

To establish the first telomere-to-telomere genome assembly of matsutake using high-fidelity long-read sequencing technology. This assembly would provide complete genome structure and intergenic sequences to facilitate future genetics, genomics, and conservation studies of this vulnerable species.

Results

A complete 161.0 Mb telomere-to-telomere assembly consisting of 13 chromosomes supported by telomeric repeats, plus a 76 kb circular mitochondrial genome, was successfully generated. A total of 21,887 protein-coding genes were predicted. Repetitive sequences (LTRs and LINEs) occupy 71.6% of the genome, with GC-rich regions enriched in LINEs suggesting possible centromeric regions.

Conclusion

This telomere-to-telomere genome assembly represents a significant advancement for matsutake research, providing complete genome structure and intergenic sequences. The assembly serves as a reference for understanding matsutake genetics, genomics, evolutionary relationships, and facilitates conservation of this vulnerable genetic resource.
  • Published in:DNA Research,
  • Study Type:Genomic sequencing and assembly,
  • Source: PMID: 37186136, DOI: 10.1093/dnares/dsad006
Scroll to Top