Adaptive evolution of Moniliophthora PR-1 proteins towards its pathogenic lifestyle

Summary

How Cacao-Attacking Fungi Evolved Special Weapons Scientists have discovered something fascinating about the fungi that cause devastating diseases in cacao trees (the source of chocolate). These fungi, called Moniliophthora, have stolen and adapted proteins from plants that were originally meant to defend against infections! The research team examined the genomes of many fungi and found that Moniliophthora species have uniquely evolved these plant-like “PR-1” proteins. While some of these proteins exist in harmless mushroom species, the disease-causing fungi have developed specialized versions that help them attack cacao trees. The study revealed that these proteins show signs of rapid evolution – evidence that they’ve been adapting to overcome the cacao tree’s defenses. Some of these proteins become especially active during infection and may help neutralize the plant’s natural antifungal compounds. This clever evolutionary strategy helps explain how these fungi became such effective pathogens, causing serious problems for chocolate production worldwide.

Background

Plant pathogenesis related-1 (PR-1) proteins belong to the CAP superfamily and have been characterized as markers of induced defense against pathogens. Moniliophthora perniciosa and Moniliophthora roreri are hemibiotrophic fungi that cause witches’ broom disease and frosty pod rot in Theobroma cacao. These fungi have a large number of plant PR-1-like genes in their genomes that are up-regulated during biotrophic interaction.

Objective

To investigate the evolution of PR-1 proteins from 22 genomes of Moniliophthora isolates and 16 other Agaricales species by performing genomic investigation, phylogenetic reconstruction, positive selection search and gene expression analysis. The study aimed to understand how these proteins adapted to the pathogenic lifestyle.

Results

Phylogenetic analysis revealed conserved PR-1 genes shared by many Agaricales saprotrophic species that diversified into new PR-1 genes putatively related to pathogenicity in Moniliophthora. PR-1 families with higher evolutionary rates showed induced expression during biotrophic interaction and evidence of positive selection, supporting adaptive changes in response to host-pathogen arms race. Although previous work showed MpPR-1 can detoxify plant antifungal compounds in yeast, only MpPR-1e, k, d were differentially expressed in response to eugenol treatment.

Conclusion

The evolution of PR-1 proteins in Moniliophthora was adaptive and potentially related to the emergence of parasitic lifestyle in this genus. The study identified specific PR-1 families putatively related to pathogenicity and host specialization, with evidence of positive selection at specific protein sites that may be important for pathogenic functions.
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