Exploring Molecular Tools for Transformation and Gene Expression in the Cultivated Edible Mushroom Agrocybe aegerita

Summary

This research developed new genetic tools for modifying an edible mushroom called Agrocybe aegerita. The scientists created methods to insert and express new genes in this mushroom, which is important for understanding how mushrooms grow and potentially improving their cultivation. Impacts on everyday life: • Could lead to improved mushroom cultivation techniques • May help develop more productive or resilient mushroom strains • Advances our understanding of how edible mushrooms grow and develop • Could contribute to better food production methods • Provides tools for future research on mushroom improvement

Background

Agrocybe aegerita is a cultivated edible mushroom that serves as a model basidiomycete for studying fruiting body formation. The researchers aimed to develop molecular tools for transformation and gene expression in this species.

Objective

To create an expandable customized molecular toolset for transformation and constitutive gene expression in Agrocybe aegerita, starting with the development of a homologous dominant marker for transformant selection.

Results

Carboxin-resistant transformants were obtained from both oidia and mycelium-derived protoplasts. Linearized plasmid DNA resulted in multiple ectopic insertions, while circular plasmid favored single integration. Gene conversion at the homologous locus was observed in one transformant. GFP fluorescence was detected with both intron-containing and intron-less constructs.

Conclusion

The study successfully developed molecular tools for transformation and gene expression in A. aegerita, demonstrating that eGFP expression is intron-independent. This advancement enables functional genetics approaches, particularly for studying fruiting-related genes.
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