Development and Optimization of Triterpenoid and Sterol Production with Ganoderma lucidum Strain G0017 in Large-Scale Liquid Fermentation

Summary

This research developed an improved method for producing valuable medicinal compounds from the Reishi mushroom in large fermentation tanks. The scientists found that carefully controlling air flow rates during fermentation significantly increased the production of beneficial compounds called triterpenoids and sterols. Impacts on everyday life: • More efficient production could make medicinal mushroom supplements more affordable • Improved manufacturing processes could lead to higher quality natural health products • The techniques developed could help produce other beneficial mushroom compounds • Advances in fermentation technology support sustainable production of natural medicines

Background

Ganoderma lucidum mycelia contain valuable active compounds like triterpenoids and sterols. However, effective submerged fermentation processes are not well documented and current yields of total triterpene and sterol remain low.

Objective

To develop and optimize a large-scale liquid fermentation process for enhanced production of triterpenoids and sterols using a newly isolated G. lucidum strain G0017, focusing on the effects of aeration rate control.

Results

The optimized process achieved triterpenoid yields of 3.34 g/L and sterol yields of 3.46 g/L, representing increases of 69.54% and 75.63% respectively compared to fixed aeration rate processes at 1.50 volume of air per volume of liquid per minute.

Conclusion

The developed multistage aeration rate control process significantly improved triterpenoid and sterol production. This optimized fermentation method shows potential for industrial-scale implementation and could be adapted to improve liquid fermentation processes for other medicinal mushrooms.
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