Comparative Transcriptome Analysis Reveals Candidate Genes Related to Cadmium Accumulation and Tolerance in Two Almond Mushroom (Agaricus brasiliensis) Strains with Contrasting Cadmium Tolerance
- Author: mycolabadmin
- 2020-09-29
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Summary
This research examined how two different strains of the almond mushroom respond differently to toxic cadmium exposure at the genetic level. The study revealed important insights into how some mushrooms can better tolerate and process environmental toxins.
Impacts on everyday life:
– Helps identify mushroom strains that accumulate less toxic metals, making them safer for consumption
– Advances understanding of how organisms can adapt to environmental pollutants
– Could lead to development of mushroom varieties better suited for growing in contaminated soils
– Provides insights for improving food safety in mushroom cultivation
– May help develop methods to use mushrooms for cleaning up metal-contaminated environments
Background
Cadmium (Cd) is a toxic metal occurring naturally in the environment. Almond mushroom (Agaricus brasiliensis) is a well-known cultivated edible and medicinal mushroom that has received increasing attention due to Cd accumulation in recent decades. The molecular mechanisms of Cd-accumulation in A. brasiliensis remain unclear.
Objective
To identify Cd-responsive genes possibly responsible for low Cd-accumulation and high Cd-tolerance by performing a comparative transcriptome analysis of two A. brasiliensis strains with contrasting Cd accumulation and tolerance – low Cd-accumulating and Cd-tolerant (J77) and high Cd-accumulating and Cd-sensitive (J1) strains.
Results
A total of 57,884 unigenes were obtained. Far fewer Cd-responsive genes were identified in J77 mycelia compared to J1 mycelia. Key differences were found in genes related to ABC transporters, ZIP Zn transporter, Glutathione S-transferase and Cation efflux family. The higher Cd-accumulation in J1 mycelia was likely due to Cd-induced upregulation of ZIP Zn transporter. Cd impaired cell wall, cell cycle, DNA replication and repair in J1, decreasing mycelium growth. J77 showed better stability of DNA replication/repair and cell wall/cycle stability.
Conclusion
The study identified comprehensive sets of DEGs influenced by Cd stress and provided insights into molecular mechanisms of A. brasiliensis Cd accumulation and tolerance. The findings revealed that J77 strain’s superior Cd tolerance was linked to better stability of DNA replication/repair systems and cell wall/cycle processes compared to J1 strain.
- Published in:PLoS One,
- Study Type:Comparative Transcriptome Analysis,
- Source: 10.1371/journal.pone.0239617