MYCOLab Ai Logo - gold and army green

Proteome Exploration to Provide a Resource for the Investigation of Ganoderma lucidum

Summary

This research provides the first detailed protein analysis of the medicinal mushroom Ganoderma lucidum, revealing hundreds of proteins that contribute to its beneficial properties. The study discovered new enzymes and compounds that could have important applications in medicine and biotechnology. Impacts on everyday life: – Identifies new proteins that could lead to development of immune-boosting supplements – Reveals enzymes that could be used in biofuel production from plant waste – Provides better understanding of how medicinal mushrooms produce their health benefits – Could help improve cultivation methods for medicinal mushrooms – May lead to new applications in natural medicine and biotechnology

Background

Ganoderma lucidum is a basidiomycete white rot fungus that has been used for medicinal purposes worldwide. While its genome and transcriptome have been recently reported, relatively little information is available at the proteomic level.

Objective

To perform a comprehensive proteomic characterization of G. lucidum at three developmental stages (16-day mycelia, and fruiting bodies at 60 and 90 days) using LC-MS/MS analysis and provide new resources for investigating this medicinal fungus.

Results

A total of 803 proteins were identified across all samples, with 247, 401 and 273 proteins detected from 16-day mycelia, 60-day and 90-day fruiting bodies respectively. Among these, 61 lignocellulose degrading proteins were detected, with most (49) found in 90-day fruiting bodies. The study also identified 14 TCA-cycle related proteins, 17 peptidases, two argonaute-like proteins, and two immunomodulatory proteins. A new immunomodulatory protein (GL18769) was successfully expressed and demonstrated high immunomodulatory activity in mouse splenic lymphocytes.

Conclusion

This first comprehensive proteomic analysis of G. lucidum revealed numerous functional proteins including wood-degrading enzymes, metabolic proteins, and bioactive compounds. The study confirmed the existence of many putative proteins predicted from the genome and provided new resources for further investigation of this medicinal fungus. The discovery and validation of a new immunomodulatory protein demonstrates the value of proteomic exploration in identifying novel bioactive compounds.
  • Published in:PLoS One,
  • Study Type:Laboratory Research,
  • Source: 10.1371/journal.pone.0119439
Scroll to Top