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Genetic Diversity Analysis of Hypsizygus marmoreus with Target Region Amplification Polymorphism

Summary

This research studied the genetic differences between different strains of an edible mushroom called Hypsizygus marmoreus using DNA analysis techniques. The findings help scientists better understand the genetic makeup of this commercially important mushroom species. Impacts on everyday life: • Helps improve mushroom cultivation techniques for better quality and yield • Enables development of new mushroom varieties with enhanced nutritional value • Supports food security through better understanding of edible mushroom genetics • Contributes to sustainable agriculture practices • Aids in quality control for commercial mushroom production

Background

Hypsizygus marmoreus is an industrialized edible mushroom that has become popular in East Asia due to its nutritional and medicinal value. While many studies have focused on its nutrient content and health benefits, genetic research has been limited. Understanding strain characteristics is crucial for production output and quality of cultivated mushrooms. Molecular markers can directly reflect genetic polymorphisms at the DNA level and are useful for analyzing genetic diversity, constructing genetic linkage maps, identifying strains, and assisting in breeding.

Objective

To evaluate the genetic diversity among 20 strains of H. marmoreus collected from different regions of China using target region amplification polymorphism (TRAP) analysis, and to sequence and analyze common TRAP fragments.

Results

The 28 TRAP primer combinations generated 287 bands, with an average of 10.27 amplified bands per primer and mean polymorphism of 69.73%. The polymorphism information content (PIC) value ranged from 0.32 to 0.50, indicating a medium level of polymorphism among strains. A total of 36 sequences were obtained from TRAP amplification, with half capable of encoding known or unknown proteins. Phylogenetic analysis classified the 20 strains into two main groups.

Conclusion

TRAP analysis was successfully applied to study genetic diversity among H. marmoreus strains, revealing a medium level of genetic diversity. The technique proved more effective than other molecular markers for evaluating genetic diversities of H. marmoreus strains. The study resulted in obtaining 36 gene sequences and provided important genetic information for strain conservation and identification.
  • Published in:The Scientific World Journal,
  • Study Type:Genetic Analysis,
  • Source: 10.1155/2014/619746
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