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Cloning and Expression Analysis of VvLcc3, a Novel and Functional Laccase Gene Possibly Involved in Stipe Elongation

Summary

This research investigated genes involved in mushroom stem growth, specifically in the paddy straw mushroom Volvariella volvacea. The scientists identified and studied a gene called Vvlcc3 that appears to help control how the mushroom’s stem elongates during development. Understanding this process is important because rapid stem growth can reduce mushroom quality and market value. Impacts on everyday life: – Could help improve cultivation methods for edible mushrooms – May lead to better quality control in commercial mushroom production – Contributes to understanding how mushrooms grow and develop – Could help reduce waste in mushroom farming – May lead to improved shelf life for harvested mushrooms

Background

Volvariella volvacea is an economically important edible mushroom typically harvested in its egg stage. The rapid transition from egg stage to elongation stage, where the stipe stretches to full length, leads to cap opening and universal veil rupture. This process negatively impacts yield and value. While stipe elongation is common in mushrooms, the mechanisms and genes involved remain poorly understood.

Objective

To identify and characterize genes related to stipe elongation in V. volvacea by analyzing laccase gene transcription patterns in stipe tissue, since some laccases have been implicated in stipe elongation in other mushroom species like Flammulina velutipes.

Results

The Vvlcc3 gene showed the highest expression among 11 laccase genes analyzed. The gene encoded a functional laccase protein of 515 amino acids containing characteristic copper-binding domains. Expression peaked during the stipe elongation stage, similar to stipe elongation-related laccases in F. velutipes. The recombinant protein showed laccase activity when expressed in P. pastoris.

Conclusion

The expression pattern and characteristics of Vvlcc3 suggest it plays a role in stipe elongation in V. volvacea, similar to laccase genes involved in stipe elongation in F. velutipes. This provides insight into the molecular mechanisms of mushroom development and could be useful for improving V. volvacea cultivation.
  • Published in:International Journal of Molecular Sciences,
  • Study Type:Molecular Biology Research,
  • Source: 10.3390/ijms161226111
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