Isolation and Characterization of Exopolysaccharide with Immunomodulatory Activity from Fermentation Broth of Morchella conica
- Author: mycolabadmin
- 2013-01-05
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Summary
This research isolated and studied a special sugar molecule from morel mushrooms grown in laboratory conditions. The molecule was found to boost immune system function by activating important immune cells. This has practical implications for human health:
• Could lead to new natural immune-boosting supplements
• Provides a sustainable way to produce beneficial compounds from rare mushrooms
• May help develop treatments for diseases affecting the immune system
• Demonstrates potential for using fermentation instead of wild mushroom harvesting
• Could reduce costs of obtaining medicinal mushroom compounds
Background
Mushroom polysaccharides have traditionally been used for preventing and treating disorders like infectious illnesses, cancers and autoimmune diseases. Morchella conica is a rare edible mushroom with proven medicinal properties, but wild specimens are difficult to culture and expensive. The fermentation broth contains similar bioactive compounds and could serve as an ideal substitute.
Objective
To isolate and characterize exopolysaccharide from submerged mycelial culture of M. conica, and evaluate its immunomodulatory activity.
Results
MCP significantly modulated nitric oxide production in macrophages and promoted splenocytes proliferation in a dose-dependent manner. Analysis showed MCP was a homogeneous mannan with an average molecular weight of 81.2 kDa. The glycosidic bond links were identified as →6)-α-D-Man p-(1→.
Conclusion
The extracted MCP demonstrated ability to modulate nitric oxide production in macrophages and promote splenocytes proliferation, suggesting it may act as a potent immunomodulatory agent. This provides a faster alternative to extracting polysaccharides from M. conica fruiting bodies.
- Published in:DARU Journal of Pharmaceutical Sciences,
- Study Type:Laboratory Research,
- Source: 10.1186/2008-2231-21-5