Genetic Analyses of the Internal Transcribed Spacer Sequences Suggest Introgression and Duplication in the Medicinal Mushroom Agaricus subrufescens

Summary

This research examined genetic variation in a medicinal mushroom species by studying specific DNA regions called ITS sequences. The scientists discovered that a French mushroom strain contained three different types of these sequences, suggesting mixing between mushroom populations from different continents. This finding helps us understand how mushroom species evolve and adapt to new environments. Impacts on everyday life: – Helps improve cultivation of medicinal mushrooms for health benefits – Provides insights into how food crops can adapt to new environments – Advances our understanding of biodiversity and species conservation – Contributes to better quality control in mushroom farming – Aids in developing more effective breeding programs for edible mushrooms

Background

The internal transcribed spacer (ITS) region of nuclear ribosomal DNA is widely used in fungal taxonomy and phylogeny due to its high success rate in PCR amplification and high degree of interspecific variation. While multicopy genes like ITS typically undergo concerted evolution making copies identical or highly similar, numerous studies have shown intra-strain and intra-species variation in various organisms.

Objective

To investigate the pattern and potential mechanism of ITS sequence heterogeneity within the wild French isolate CA487 of Agaricus subrufescens, which showed notable ITS sequence heterogeneity in previous analysis.

Results

Three distinct types of ITS sequences (A, B, and C) were identified with balanced distribution, differing at 13 polymorphic positions. Phylogenetic comparisons revealed type C sequence was similar to Oceanian and Asian specimens while types A and B were close to Americas/European specimens. Genetic analyses showed types A and B were alleles segregating at one locus ITSI, while type C was located at another unlinked locus ITSII and present in only one of two constitutive haploid nuclei of the parent CA487.

Conclusion

The findings suggest a relatively recent introduction of the type C sequence and duplication of the ITS locus in strain CA487. The presence of three ITS types indicates potential hybridization between geographically distinct populations of A. subrufescens. This study reveals complex evolutionary dynamics involving introgression and gene duplication in fungal ribosomal DNA regions.
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