Diverse, Novel Mycoviruses from the Virome of a Hypovirulent Sclerotium rolfsii Strain

Summary

This research discovered multiple new viruses that infect a plant-damaging fungus called Sclerotium rolfsii. These viruses can weaken the fungus’s ability to cause disease in plants, potentially offering a natural way to protect crops. The study provides important insights into how these beneficial viruses could be used to control harmful plant diseases. Impacts on everyday life: – Could lead to more environmentally friendly methods of protecting food crops – May reduce the need for chemical fungicides in agriculture – Helps understand how viruses can be used beneficially in plant disease management – Could improve food security by providing new tools to fight crop diseases – May lead to lower food production costs through natural disease control

Background

Sclerotium rolfsii is a devastating plant pathogen that causes southern blight disease in over 600 plant species worldwide. Mycoviruses that induce hypovirulence in plant pathogenic fungi show potential as biological control agents. However, mycoviruses are rarely reported in S. rolfsii.

Objective

To characterize the complex virome from a hypovirulent S. rolfsii strain and identify diverse viral segments using high-throughput transcriptome sequencing. The study aimed to clarify the species and genomes of all mycoviruses infecting the hypovirulent strain BLH-1 and determine potential interactions between RNA silencing machinery and mycoviruses in S. rolfsii.

Results

The study revealed that the hypovirulent strain harbors various new viral species showing affinity to the families Benyviridae, Endornaviridae, Fusariviridae, Hypoviridae, and Fusagraviridae. Some viral sequences could not be assigned to existing families and showed similarities to Alphavirus, Ourmiavirus, phlegivirus-like and Curvularia thermal tolerance virus-like groups. The results indicated that Dicer-mediated gene silencing mechanism was present in S. rolfsii.

Conclusion

This is the first comprehensive analysis of viral diversity in a hypovirulent S. rolfsii strain. The findings provide insight into mycovirus taxonomy and evolution while suggesting potential applications for biocontrol of fungal plant diseases. The discovery of novel mycoviruses with diverse molecular and biological properties advances understanding of viral ecology and evolution.
Scroll to Top