Genome and Secretome of Chondrostereum purpureum Correspond to Saprotrophic and Phytopathogenic Life Styles
- Author: mycolabadmin
- 2019-03-01
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Summary
This research reveals how a fascinating fungus called Chondrostereum purpureum can both decompose dead wood and act as a plant pathogen. The scientists decoded its genetic makeup and studied the proteins it produces, showing it has an impressive arsenal of enzymes that allow it to break down tough plant materials and switch between different lifestyles. This has important implications for both forest management and biotechnology.
Key impacts on everyday life:
– Helps explain how fungi can be used to naturally control unwanted tree growth in forests and urban areas
– Provides insights for developing more efficient ways to break down plant waste into useful products
– Advances our understanding of plant diseases and how to potentially control them
– Identifies enzymes that could be useful for industrial applications like biofuel production
– Demonstrates nature’s sophisticated solutions for recycling plant materials in ecosystems
Background
Chondrostereum purpureum (Silverleaf fungus) is both a saprotrophic fungus and plant pathogen that is commercially used to control forest ‘weed’ trees in vegetation management. However, little is known about its lignocellulose-degrading capabilities and enzymatic machinery responsible for its degradative potential. It remains unclear which group of wood-rot fungi it belongs to.
Objective
To sequence and analyze the draft genome of C. purpureum and perform quantitative proteomic analysis during growth in different culture conditions to understand its enzymatic machinery and degradative capabilities. The study aimed to characterize the fungus’s lignocellulolytic enzymes and determine its classification among wood-rot fungi.
Results
The draft genome of C. purpureum was 41.2 Mbp and contained characteristic lignocellulolytic hydrolases and oxidoreductases. The fungus showed high abundance of certain genes through gene expansion, including 45 laccase genes and nine GH7 genes. The fungus responded to complex growth substrates by secreting various lignocellulolytic and lignin-modifying enzymes. Phylogenetic analysis placed C. purpureum in a clade with other pathogenic fungi within the Agaricales order.
Conclusion
Based on genetic and enzymatic constitution, C. purpureum is classified as a ‘phytopathogenic’ white-rot fungus that possesses a complex extracellular enzyme machinery enabling efficient lignocellulose degradation during both saprotrophic and phytopathogenic life phases. The fungus shows characteristics of both white-rot fungi and plant pathogens, with expanded gene families related to both lifestyles.
- Published in:PLoS One,
- Study Type:Genomic and Proteomic Analysis,
- Source: 10.1371/journal.pone.0212769