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Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

Summary

This research developed a genetic marker to identify different growth patterns in the edible black wood ear mushroom. This advancement helps mushroom farmers and breeders select and grow better strains more efficiently. Impacts on everyday life: • Improved cultivation efficiency for mushroom farmers • Better quality control in mushroom production • More consistent supply of edible mushrooms for consumers • Lower production costs that may lead to more affordable mushrooms • Advanced breeding techniques that could be applied to other agricultural products

Background

Auricularia auricula-judae is a wood rot fungus commonly known as black wood ear in China. It has been cultivated for over 2100 years and is currently the second most popular edible fungus in China, producing 98% of global output. The fruiting body pattern is an important agronomic trait and breeding target, with two main types: cluster type (CL) and chrysanthemum type (CH). CL strains are preferred as they are easier to cultivate and propagate compared to CH strains.

Objective

To develop sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern in A. auricula-judae based on sequence-related amplified polymorphism (SRAP) markers. This would enable early identification of cluster-type strains for breeding purposes.

Results

Ten different bands (189-522 bp) were amplified using SRAP primers. A single SCAR marker named ‘SCL-18’ was developed that produced a 522-bp band specific to cluster-type strains but not chrysanthemum strains. This marker successfully distinguished between the two fruiting body patterns in verified strains.

Conclusion

The researchers successfully developed a stable SCAR marker (SCL-18) that is closely associated with the cluster-type fruiting body trait in A. auricula-judae. This marker provides an effective method for early identification of cluster-type strains and will be useful for molecular marker-assisted breeding programs.
  • Published in:Mycobiology,
  • Study Type:Laboratory Research,
  • Source: 10.1080/12298093.2018.1454004
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