A Genetic Linkage Map of Pleurotus tuoliensis Integrated with Physical Mapping of the De Novo Sequenced Genome and the Mating Type Loci
- Author: mycolabadmin
- 2018-01-05
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Summary
This research provides the first detailed genetic map and genome sequence of Bailinggu, an important cultivated mushroom in China. The study helps scientists understand the genetic makeup of this mushroom species, which is crucial for developing improved varieties. This impacts everyday life in several ways:
• Better mushroom varieties could lead to more affordable and higher quality mushrooms for consumers
• Improved disease resistance could reduce the need for chemical treatments in mushroom cultivation
• More efficient cultivation methods could make mushroom farming more profitable and sustainable
• Enhanced understanding of mushroom genetics helps preserve and improve food security
• Scientific advances in mushroom breeding contribute to agricultural innovation and food production
Background
Pleurotus tuoliensis (Bailinggu) is a commercially cultivated white mushroom species with increasing popularity in China and other Asian countries. Commercial profits are currently low due to low yield, long cultivation period and disease sensitivity. The species requires breeding efforts to improve important agronomical traits, starting with developing saturated genetic linkage and physical maps to enable genetic and molecular breeding approaches.
Objective
To construct a genetic linkage map for P. tuoliensis and integrate it with physical mapping of the de novo sequenced genome and mating type loci to provide a basis for marker-assisted breeding.
Results
The genetic map covers 1073 cM with average marker spacing of 1.0 cM. The genome was assembled to 40.8 Mb consisting of 500 scaffolds. 97.4% of SNP markers were physically localized on 78 scaffolds, covering 33.9 Mb (83.1%) of the whole genome. The genome showed high colinearity with P. eryngii var. eryngii. Five putative pheromone receptors and two putative pheromone precursors were identified for the mating type B locus.
Conclusion
This study produced the first genetic linkage map integrated with physical mapping of the de novo sequenced genome and mating type loci of P. tuoliensis. The assembled genome and genetic map provide a foundation for marker-assisted breeding of this economically important mushroom species.
- Published in:BMC Genomics,
- Study Type:Genomic Analysis,
- Source: 10.1186/s12864-017-4421-z