The Genetic Linkage Map of the Medicinal Mushroom Agaricus subrufescens Reveals Highly Conserved Macrosynteny with the Congeneric Species Agaricus bisporus

Summary

This research created the first genetic map of the medicinal mushroom Agaricus subrufescens and showed that its genome structure is very similar to the common button mushroom. This discovery has important implications for mushroom breeding and cultivation. Impacts on everyday life: – Helps develop better strains of medicinal mushrooms for health benefits – Enables more efficient cultivation methods for mushroom farmers – Could lead to improved mushroom varieties with better taste and nutrition – Supports sustainable agriculture through better mushroom breeding – May help reduce costs of medicinal mushroom products for consumers

Background

Agaricus subrufescens is an emerging medicinal mushroom crop known for its therapeutic properties and potential medicinal applications. While A. bisporus (button mushroom) is well-studied as a model for edible mushrooms, A. subrufescens lacks genetic and molecular tools for research and breeding.

Objective

To develop the first genetic linkage map for A. subrufescens and analyze the syntenic relationships between A. subrufescens and A. bisporus genomes to better understand their evolutionary relationship and enable knowledge transfer between species.

Results

The map covers 1701 cM with an average marker spacing of 8.2 cM. Using 96 homologous loci, researchers demonstrated high macrosynteny with A. bisporus genome. The 13 main linkage groups of A. subrufescens were syntenic to the 13 A. bisporus chromosomes. Electronic mapping showed homologous loci were evenly spread except for some local hot/cold spots. The MAT locus was located on a chromosome homologous to that carrying the MAT locus in A. bisporus.

Conclusion

The study provides the first genetic linkage map for A. subrufescens and demonstrates extensive conservation of synteny with A. bisporus. This map provides a framework for future genetic studies and breeding programs. The high synteny between species suggests potential for knowledge transfer from the model species A. bisporus to A. subrufescens.
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