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Optimization of Mycelia Selenium Polysaccharide Extraction from Agrocybe cylindracea SL-02 and Assessment of their Antioxidant and Anti-ageing Activities

Summary

This research explored how to optimize the extraction of selenium-enriched compounds from mushroom mycelia and tested their potential anti-aging benefits. The study found that these compounds had strong antioxidant properties and could help prevent aging-related damage in mice. This has important implications for everyday life: • Could lead to new natural dietary supplements for healthy aging • Provides a safer alternative to synthetic antioxidants • Offers a new way to address selenium deficiency through mushroom-based products • May help develop functional foods that protect against oxidative stress • Demonstrates potential for using mushrooms as natural medicine sources

Background

Ageing is an inevitable process that damages cell structure and promotes physiological dysfunction. Free radical theory suggests oxidative stress plays a vital role in ageing. While synthetic antioxidants have side effects, there is growing interest in natural antioxidants from edible materials. Agrocybe cylindracea is a precious edible and medicinal mushroom with various beneficial activities. Selenium, an essential trace element involved in antioxidant enzymes, is often deficient in common foods. Organic selenium from mushrooms has higher bioavailability and lower toxicity compared to inorganic forms.

Objective

To optimize the purification conditions for mycelia selenium polysaccharides (MSPS) from Agrocybe cylindracea SL-02 and evaluate their antioxidant and anti-ageing properties both in vitro and in vivo.

Results

The optimum extraction conditions were: temperature 94.99°C, pH 9, and precipitation temperature 12°C, yielding 11.036% MSPS. MSPS showed superior antioxidant activities compared to MPS in all in vitro assays. In vivo studies demonstrated MSPS significantly reduced MDA and cholesterol levels while improving SOD, GSH-Px and T-AOC activities in D-galactose treated mice. The selenium content was 1.76 mg/g at 6 μg/mL Na2SeO3 concentration.

Conclusion

MSPS from Agrocybe cylindracea demonstrates potent antioxidant and anti-aging properties both in vitro and in vivo. The selenium-enriched mycelia represent a promising dietary source of bioavailable selenium with potential applications as functional foods and natural drugs for preventing aging induced by toxic chemicals.
  • Published in:PLOS One,
  • Study Type:Laboratory Research,
  • Source: 10.1371/journal.pone.0160799
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