Antioxidant Properties of Mushroom Mycelia Obtained by Batch Cultivation and Tocopherol Content Affected by Extraction Procedures

Summary

This research explored how to efficiently produce and extract beneficial compounds from mushroom mycelia grown in laboratory conditions. The study found that different extraction methods can affect which healthy compounds are obtained from the mushrooms, particularly discovering high levels of vitamin E (tocopherols) using a special extraction technique. This has important implications for developing natural health supplements and functional foods. Impacts on everyday life: – Provides new ways to produce natural antioxidant supplements – Could lead to better quality and more effective mushroom-based health products – Demonstrates potential for developing new functional foods with enhanced health benefits – Offers natural alternatives for preventing oxidative stress-related conditions – Shows promise for creating anti-inflammatory supplements from mushroom sources

Background

Medicinal mushrooms like P. ostreatus and C. comatus are used as food sources and contain therapeutic biomolecules. Their mycelia can be obtained through fermentation to produce biologically active supplements. The extractive efficiency depends on species, nutrient sources, and cultivation parameters. Mycelia extracts prevent free radical damage and protect against cellular malignization. The presence of tocopherols helps protect LDL against oxidative stress and high plasma cholesterol levels.

Objective

The aim was to assess the antioxidant activity of lyophilized mycelia from 5 strains of P. ostreatus and C. comatus obtained through batch cultivation and fluidized bed extraction. The study sought to correlate and identify key molecules determining antioxidative activities through HPLC chromatographic analysis.

Results

The study found significant variations in biomass productivity and antioxidant compound accumulation between strains. C. comatus M8102 showed the highest biomass productivity at 45.37 g/L/day. The highest phenolic content was found in P. ostreatus PSI101109 at 98.60 mg/g biomass. Notable amounts of α-tocopherol were detected, with C. comatus M8102 showing the highest content at 179.51 mg/100g extract. A novel finding was the presence of tocopherol acetate in P. ostreatus PQMZ91109 extract at 4.8 mg/100g.

Conclusion

The bioreactor cultivation of mycelia proved to be a reproducible method for obtaining extracts with high biological activity. The fluidized bed extraction process favored the presence of tocopherols, representing a novel finding compared to previous studies. The extraction method could modulate the composition of active compounds in the final extract. The mycelia extracts showed significant antioxidant and anti-inflammatory properties, suggesting potential use as ingredients in functional products.
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