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Improvement of Triterpenoid Production in Mycelia of Antrodia Camphorata Through Mutagenesis Breeding and Amelioration of CCL4-Induced Liver Injury in Mice

Summary

This research focused on improving the production of beneficial compounds from a medicinal mushroom called Antrodia camphorata. Scientists created an enhanced strain that produces significantly more therapeutic compounds that can protect the liver from damage. This advancement makes it more feasible to develop medicines from this traditionally used fungus. Impacts on everyday life: – Makes natural liver-protecting medicines more accessible and affordable – Provides a sustainable source of valuable medicinal compounds – Advances the development of natural alternatives to conventional drugs – Demonstrates how traditional medicines can be improved through modern biotechnology – Could lead to new treatments for liver diseases

Background

Antrodia camphorata is a medicinal mushroom that produces bioactive triterpenoids used in traditional Chinese medicine to treat various illnesses. Due to its limited availability in the wild and strict growing requirements, there is a need to improve triterpenoid yields through submerged fermentation. Current production levels are low, hampering clinical applications.

Objective

To generate high triterpenoid-producing A. camphorata strains through random mutagenesis breeding and optimize culture conditions to improve yields. Additionally, to evaluate the hepatoprotective effects of the extracted triterpenoids against CCl4-induced liver injury in mice.

Results

The mutant strain E3-64 produced 255.5 mg triterpenoids per gram dry mycelia under optimized conditions, a 137.7% increase compared to the parent strain. The mutant showed increased expression of key genes involved in triterpenoid biosynthesis and contained different genome-wide SNPs. The mutant produced 94% and 178% more antcin A and antcamphin A respectively, while producing less antcin B and G. The extracted triterpenoids showed significant hepatoprotective effects against CCl4-induced liver injury in mice.

Conclusion

The study successfully generated an A. camphorata mutant with significantly improved triterpenoid production through mutagenesis breeding and culture optimization. The mutant E3-64 achieved the highest reported triterpenoid yield to date in flask cultures. The extracted triterpenoids demonstrated excellent hepatoprotective activity, showing promise for commercial applications.
  • Published in:Heliyon,
  • Study Type:Laboratory Research,
  • Source: 10.1016/j.heliyon.2023.e19621
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