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The Integration of Metabolome and Proteome Reveals Bioactive Polyphenols and Hispidin in ARTP Mutagenized Phellinus baumii

Summary

This research focused on improving a medicinal mushroom called Phellinus baumii to enhance its health-promoting properties. Scientists used a special mutation technique to create an improved strain that produces more beneficial compounds, particularly antioxidants and anti-cancer substances. The study helps understand how to make natural medicines more effective. Impacts on everyday life: • Provides a way to make natural medicines more potent and effective • Offers potential new treatments for cancer using natural compounds • Demonstrates how traditional medicines can be improved through modern science • Could lead to more affordable and accessible natural health products • Helps validate the use of traditional Asian medicinal mushrooms in modern healthcare

Background

Phellinus baumii, known as ‘Sang Huang’ in China and ‘Meshimakobu’ in Japan, is a widely used medicinal mushroom in Asia valued for its health benefits and biological activities. It grows on mulberry trees and has traditionally been used as both a folk medicine and food source. Previous studies have shown it possesses anti-tumor, anti-inflammatory, anti-oxidation and anti-infection properties.

Objective

To generate and characterize an improved P. baumii strain with enhanced biological activities through ARTP mutagenesis, and to understand the underlying metabolic mechanisms through integrated metabolomic and proteomic analysis.

Results

The mutant strain A67 showed significantly higher polyphenol and flavonoid content compared to wild type, with 1.33 and 1.87-fold increases respectively. A67 demonstrated enhanced anti-tumor activity against HepG2 and K562 cells and stronger antioxidant properties. Metabolomic analysis revealed increased production of bioactive compounds, particularly hispidin. Proteomic analysis showed upregulation of the phenylpropanoid biosynthesis pathway enzymes PAL, C4H and 4CL, explaining the increased polyphenol production.

Conclusion

The study successfully generated an improved P. baumii strain A67 through ARTP mutagenesis with enhanced production of bioactive polyphenols, particularly hispidin. The integrated metabolomic and proteomic analysis revealed that upregulation of the phenylpropanoid pathway was responsible for the increased production of bioactive compounds and enhanced biological activities.
  • Published in:Scientific Reports,
  • Study Type:Experimental Research,
  • Source: 10.1038/s41598-019-52711-7
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