Bioactive Compounds of Ganoderma boninense Inhibited Methicillin-Resistant Staphylococcus aureus Growth by Affecting Their Cell Membrane Permeability and Integrity

Summary

This research discovered that compounds from the fungus Ganoderma boninense can effectively kill antibiotic-resistant bacteria (MRSA) by destroying their cell membranes. This is significant because MRSA infections are increasingly difficult to treat with conventional antibiotics. Impacts on everyday life: – Provides a potential new source of antibiotics to fight drug-resistant infections – Demonstrates the value of studying fungi for developing new medicines – Could lead to more effective treatments for dangerous bacterial infections – Shows promise for developing alternatives to current antibiotics – Highlights the importance of preserving fungal species for medical research

Background

Some species of Ganoderma, such as G. lucidum, are well-known as traditional Chinese medicine (TCM), and their pharmacological value was scientifically proven in modern days. However, G. boninense is recognized as an oil palm pathogen, and its biological activity is scarcely reported.

Objective

This study aimed to investigate the antibacterial properties of G. boninense fruiting bodies, which formed by condensed mycelial, produced numerous and complex profiles of natural compounds.

Results

Strong susceptibility was observed in methicillin-resistant Staphylococcus aureus (MRSA) in elute fraction with zone inhibition of 41.08 ± 0.04 mm and MIC value of 0.078 mg mL−1. A total of 23 peaks were detected using MS, with eight compounds showing known antimicrobial activity including aristolochic acid, aminoimidazole ribotide, lysine sulfonamide 11v, carbocyclic puromycin, fenbendazole, acetylcaranine, tigecycline, and tamoxifen.

Conclusion

G. boninense extract induces irreversible damage to the cell membrane of MRSA, thus causing cellular lysis and death. The antibacterial potency of this semi-purified fraction shows potential for development of new therapeutic agents.
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