Highly Regioselective Biotransformation of Ginsenoside Rb2 into Compound Y and Compound K by β-Glycosidase Purified from Armillaria mellea Mycelia
- Author: mycolabadmin
- 2017-07-08
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Summary
This research discovered a new way to make ginseng compounds more beneficial for human health using enzymes from mushrooms. Scientists found that an enzyme from the honey fungus mushroom can transform a common ginseng compound into more easily absorbed and potentially more effective forms.
Impacts on everyday life:
• Could lead to more effective ginseng supplements
• May improve the absorption of ginseng’s beneficial compounds in the body
• Demonstrates a natural way to enhance herbal medicine effectiveness
• Could reduce the cost of producing beneficial ginseng compounds
• Shows potential for using mushroom enzymes in other natural product improvements
Background
Ginseng saponins (ginsenosides) have biological activities that depend on the type, number, and position of sugar molecules linked to their core structure. Minor ginsenosides, which are deglycosylated forms, show greater biological activity than major ginsenosides but are nearly absent in unprocessed ginseng. Converting major ginsenosides into minor forms through deglycosylation could enhance their bioactive effects.
Objective
To transform ginsenoside Rb2, a major protopanaxadiol-type ginsenoside, into minor ginsenosides using β-glycosidase (BG-1) purified from Armillaria mellea mycelium and characterize the transformation process.
Results
BG-1 first hydrolyzed the 3-O-outer β-d-glucoside of Rb2, then the 3-O-β-d-glucoside of C-O to form C-Y. With prolonged reaction time, C-Y was gradually converted to C-K. The pathway Rb2 → Rd → F2 → C-K was not observed. Optimal reaction conditions were pH 4.0-4.5, temperature 45-60°C, and reaction time 72-96 hours.
Conclusion
β-Glycosidase purified from A. mellea mycelium can effectively transform ginsenoside Rb2 into compounds Y and K. This represents the first reported transformation of Rb2 into C-Y and C-K using an enzyme from basidiomycete mushroom.
- Published in:Journal of Ginseng Research,
- Study Type:Laboratory Research,
- Source: 10.1016/j.jgr.2017.07.001