A Second HD Mating Type Sublocus of Flammulina velutipes is at Least Di-allelic and Active: New Primers for Identification of HD-a and HD-b Subloci
- Author: mycolabadmin
- 2019-02-22
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Summary
This research provides important insights into how the winter mushroom (Flammulina velutipes) controls its mating and reproduction at the genetic level. The study discovered and characterized a previously unclear genetic region that plays an active role in determining mating compatibility between different strains of this mushroom species. The findings have practical applications for mushroom breeding and production.
Impacts on everyday life:
– Improved breeding techniques for commercial mushroom production
– More efficient cultivation of winter mushrooms for food industry
– Better understanding of fungal reproduction for agricultural applications
– Development of new tools for mushroom strain identification
– Enhanced ability to produce hybrid mushroom varieties
Background
Sexual development in Flammulina velutipes is controlled by two different mating type loci (HD and PR). The HD locus contains homeodomain (Hd) genes on two separate HD subloci: HD-a and HD-b. While the functionality of the HD-b sublocus has been largely confirmed, the status and content of the HD-a sublocus has remained unclear.
Objective
To examine the function of the HD-a sublocus by analyzing genome sequences of F. velutipes strains and testing through series of amplification by specific primer sets. Additionally, to confirm activity of di-allelic HD-a locus through crossing strains with different combinations of HD-a and HD-b subloci.
Results
Sublocus HD-b contained various fixed Hd1/Hd2 gene pairs, while HD-a sublocus contained either a conserved Hd2 gene or a newly discovered conserved Hd1 gene. Strains with similar HD-b subloci could form normal dikaryons if their HD-a subloci differed, indicating that the HD-a sublocus is at least di-allelic and actively involved in mating type compatibility. New primer sets were developed that specifically amplify HD-a or HD-b subloci in the majority of F. velutipes strains.
Conclusion
The HD-a sublocus is confirmed to be active and di-allelic in F. velutipes. The newly developed primer sets allow quick identification of unknown HD mating types and confirmation of HD mating type compatibility by PCR in F. velutipes strains.
- Published in:PeerJ,
- Study Type:Genomic Analysis,
- Source: 10.7717/peerj.6292