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Transcriptional Dynamics of Genes Involved in Meiosis, Carbohydrate, and Secondary Metabolism During Sporulation in Ganoderma lucidum

Summary

This research examined how genes are activated during spore formation in the medicinal mushroom Ganoderma lucidum. The study revealed important changes in metabolism and gene regulation that occur as the mushroom produces spores. Understanding these processes could help improve cultivation methods and medicinal compounds production. Impacts on everyday life: • Could lead to improved production of medicinal mushroom supplements • May help develop better methods for growing medicinal mushrooms • Could enable more efficient extraction of beneficial compounds • May lead to enhanced quality control in mushroom-based products • Could contribute to developing new therapeutic applications

Background

Ganoderma lucidum spores (GLS) are mature germ cells ejected from the abaxial side of the pileus and have diverse pharmacological effects. While GLS contain bioactive components and unique metabolites compared to other parts of G. lucidum, the genetic regulation of sporulation in this medicinal mushroom remains poorly understood.

Objective

To analyze the transcriptional dynamics during G. lucidum sporulation by examining gene expression profiles at three sequential developmental stages of spore formation, focusing on genes involved in meiosis, carbohydrate metabolism, and secondary metabolism.

Results

The study identified 1598 differentially expressed genes across the developmental stages. Genes related to carbohydrate metabolism were strongly expressed during spore morphogenesis, particularly those involved in trehalose and malate synthesis. Genes involved in triterpenoid and ergosterol biosynthesis showed high expression in young fruiting bodies but decreased during sporulation. Twenty-four transcription factors were found to be developmentally regulated, including several zinc finger proteins potentially involved in sporulation control.

Conclusion

The research provides comprehensive transcriptomic data revealing dynamic gene expression patterns during G. lucidum sporulation. Key findings include the upregulation of carbohydrate metabolism genes, downregulation of secondary metabolite biosynthesis, and identification of specific transcription factors involved in spore development. These results enhance understanding of basidiosporogenesis in G. lucidum and provide valuable targets for spore yield breeding.
  • Published in:Genes (Basel),
  • Study Type:Transcriptome Analysis,
  • Source: 10.3390/genes12040504
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