Integrated Metabolomics and Transcriptomics Unravel the Metabolic Pathway Variations for Different Sized Beech Mushrooms

Summary

This research examined how the size of beech mushrooms affects their nutritional and flavor properties by studying their molecular composition and gene activity. The study found that mushroom size significantly influences nutrient content and taste characteristics, with smaller mushrooms having different compounds than larger ones. This has practical implications for both consumers and producers. Impacts on everyday life: • Helps consumers choose mushrooms based on their preferred taste profile – smaller ones having more bitter-umami taste while larger ones have more sweet-umami flavor • Enables mushroom farmers to optimize harvest timing based on desired nutritional properties • Provides guidance for food manufacturers in selecting mushroom sizes for different culinary applications • Helps improve cultivation methods to enhance specific nutritional or medicinal properties • Allows for better quality control in commercial mushroom production

Background

Beech mushrooms (Hypsizygus marmoreus) are valued in East Asian societies for their earthy flavor, chewy texture, and nutritional properties. While these characteristics are influenced by the mushroom’s metabolome and transcriptome during growth and development, the underlying molecular mechanisms governing spatial distribution of nutrients and bioactive compounds across the fruiting body remain largely unknown.

Objective

This study aimed to conduct integrated metabolomic and transcriptomic profiling of different sized beech mushrooms across spatial components (cap and stipe) to delineate their signature metabolic pathways and understand how size affects nutritional and functional properties.

Results

Small-sized mushrooms showed higher abundance of amino acids, nucleotides, and terpenoid metabolism-related metabolites and genes. Carbohydrates and TCA intermediate metabolites, along with corresponding genes, increased linearly with mushroom size. Flavor-related metabolites varied across different sized mushrooms. The study revealed distinct metabolic states and pathway regulations during mushroom development stages.

Conclusion

The research demonstrated that beech mushrooms develop fruiting bodies through upregulation of amino acid, nucleotide, and terpenoid metabolism in early stages, while later development involves increased carbohydrate metabolism and TCA cycle activity. These metabolic variations influence the mushrooms’ nutritional, functional, and sensory characteristics across different sizes.
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