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iTRAQ-Based Quantitative Proteomic Analysis Reveals Proteomic Changes in Mycelium of Pleurotus ostreatus in Response to Heat Stress and Subsequent Recovery

Summary

This research investigated how oyster mushrooms respond and adapt to high temperatures at the molecular level. The study revealed complex cellular mechanisms that allow mushroom cells to survive heat stress and recover afterward. Understanding these processes is crucial for improving mushroom cultivation in warm conditions. Impacts on everyday life: – Helps improve commercial mushroom production in warm climates – Provides insights for developing more heat-resistant mushroom strains – Contributes to food security by protecting mushroom crops from heat damage – Demonstrates natural stress adaptation mechanisms that could inspire biotechnology applications – Supports sustainable agriculture by optimizing growing conditions

Background

High temperature is a key limiting factor for mycelium growth and development in Pleurotus ostreatus (oyster mushroom). Thermotolerance includes both the direct response to heat stress and the ability to recover from heat stress. Understanding the mechanisms of thermotolerance is important as P. ostreatus is the third largest edible fungus produced in China and often encounters heat stress during cultivation which reduces growth and production.

Objective

To better understand the mechanism of thermotolerance in P. ostreatus by analyzing morphological and physiological changes combined with an iTRAQ-based proteomics analysis of P. ostreatus subjected to heat stress (40°C for 48h) followed by recovery at 25°C for 3 days.

Results

High temperature increased TBARS concentrations indicating mycelial membrane damage, but these changes returned to control levels during recovery. A total of 204 proteins showed differential expression during heat stress and/or recovery phase. Of these, 47 proteins responded to both stress and recovery conditions, while 84 and 73 proteins were responsive to only heat stress or recovery conditions respectively. Key proteins involved in carbohydrate/energy metabolism, signal transduction, and protein metabolism formed three heat stress response networks.

Conclusion

The study revealed that effective regulatory protein expression related to MAPK-pathway, antioxidant enzymes, heat shock proteins, and glycolysis play important roles in enhancing P. ostreatus adaptation to and recovery from heat stress. The diverse array of proteins affected indicates remarkable flexibility in mycelium metabolism contributing to heat stress survival. Novel heat-responsive proteins like PAL, LDS and MAPK were identified, providing new insights into thermotolerance mechanisms in basidiomycetes.
  • Published in:Frontiers in Microbiology,
  • Study Type:Laboratory Research,
  • Source: 10.3389/fmicb.2018.02368
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