Isolation, Characterization, and Metabolic Profiling of Ceratorhiza hydrophila from the Aquatic Plant Myriophyllum spicatum

Author: mycolabadmin
2022-04-21
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Summary

This research discovered and characterized a fungus called Ceratorhiza hydrophila from an aquatic plant in Egypt’s Nile River. The study revealed this fungus produces useful enzymes and compounds that could have practical applications in medicine and industry. Impact on everyday life: • Could lead to new natural antibiotics to fight bacterial infections • May provide sustainable sources of industrial enzymes for products like detergents and food processing • Offers potential new compounds for pharmaceutical drug development • Contributes to understanding aquatic ecosystem relationships • May help develop eco-friendly alternatives to synthetic chemicals

Background

Aquatic habitats are rich environments for microorganisms. Ceratorhiza hydrophila is an anamorphic basidiomycetous species belonging to the Ceratobasidiaceae family that infects aquatic or semi-aquatic plants. While there is limited data on its biotechnological potential, related basidiomycetous species have shown ability to produce various metabolic products including amino acids, fatty acids, sugar alcohols, monosaccharides and carboxylic acids.

Objective

To investigate the antibacterial properties, enzyme production capabilities, and metabolic profile of a newly isolated Ceratorhiza hydrophila strain from the submerged aquatic plant Myriophyllum spicatum. The study aimed to characterize the fungus morphologically and through DNA sequencing while exploring its potential as a source of antimicrobial agents and essential metabolites.

Results

The fungus was successfully identified as Ceratorhiza hydrophila isolate EG19 (GenBank ID: MK387081). It demonstrated ability to produce protease and cellulase enzymes. The fungal extract showed intermediate antibacterial activity against Gram-positive bacteria including Streptococcus pneumonia, Micrococcus luteus, and Staphylococcus aureus. NMR analysis identified 23 metabolites including amino acids (31%), sugars (9%), amines (9%), sugar alcohols (4%), and alkaloids (4%).

Conclusion

This is the first report of C. hydrophila isolation from M. spicatum in Egypt and the first characterization of its enzymatic capabilities and metabolic profile. The fungus shows promise as a source of antimicrobial agents and essential metabolites, with potential applications in various industries. The metabolic findings provide valuable information for species classification and chemotaxonomy.

Published in:Mycobiology,
Study Type:Laboratory Research,
Source: 10.1080/12298093.2022.2059889