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Enhanced Expression of Thaumatin-Like Protein Gene (LeTLP1) Endows Resistance to Trichoderma atroviride in Lentinula edodes

Summary

This research discovered how shiitake mushrooms naturally defend themselves against harmful mold infections. Scientists identified a specific protein gene (LeTLP1) that helps protect mushrooms from a destructive green mold. When this protective gene is more active, mushrooms show better resistance to mold infection. Impacts on everyday life: – Potential for developing more disease-resistant shiitake mushroom varieties – Could lead to reduced use of chemical fungicides in mushroom farming – May help increase mushroom crop yields and quality – Could result in more affordable and higher quality shiitake mushrooms for consumers – Demonstrates natural ways to protect food crops without chemicals

Background

Lentinula edodes (shiitake mushrooms) is heavily affected by Trichoderma atroviride infection, causing yield loss and decreased quality. The fungus competes for resources and space, harming shiitake cultivation. While fungicides can control green mold disease, increasing resistance and residue concerns have sparked interest in alternative control methods.

Objective

To identify and characterize resistance mechanisms against T. atroviride in L. edodes by comparing resistant and susceptible strains, with focus on the role of the thaumatin-like protein gene LeTLP1.

Results

The study identified strain Y3334 as highly resistant and Y55 as susceptible to T. atroviride. Transcriptome analysis revealed LeTLP1 was significantly upregulated in the resistant strain during infection. Overexpression of LeTLP1 enhanced resistance while silencing reduced it. The purified LeTLP1 protein showed direct antifungal activity against T. atroviride, with the Y3334 variant being more effective than the Y55 variant.

Conclusion

Enhanced expression of the thaumatin-like protein gene LeTLP1 plays a crucial role in L. edodes resistance against T. atroviride infection. This finding provides insights into molecular mechanisms of fungal resistance and offers potential applications for breeding resistant strains.
  • Published in:Life (Basel),
  • Study Type:Experimental Research,
  • Source: 10.3390/life11080863
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