Polyphasic Systematics of the Fungicolous Genus Cladobotryum Based on Morphological, Molecular and Metabolomics Data
- Author: mycolabadmin
- 2022-08-20
- View Source
Summary
This research focused on studying and classifying different species of Cladobotryum fungi using multiple scientific approaches. These fungi are important because they grow on other fungi and can affect mushroom cultivation. The researchers used advanced techniques including microscopic examination, DNA analysis, and chemical profiling to better understand and classify these organisms.
Impacts on everyday life:
– Helps protect commercial mushroom cultivation from fungal diseases
– Contributes to better understanding of natural fungal interactions in ecosystems
– May lead to development of new biological control methods for plant diseases
– Improves food security by protecting cultivated mushrooms
– Advances scientific methods for identifying and classifying fungi that affect agriculture
Background
Species of the anamorphic genus Cladobotryum are known for their fungicolous lifestyle, making them important mycopathogens in fungiculture. Many morphological, ecological, and molecular phylogenetic studies of the genus have been done to date, but taxonomic uncertainties and challenges still remain. Fungal secondary metabolites, being vastly diverse, are utilized as an extra tool in fungal systematics. Despite being studied for their potentially bioactive compounds, Cladobotryum species are insufficiently investigated regarding metabolomics.
Objective
The aim of this study is the identification of Greek strains of Cladobotryum by integrating morphological data, ITS-based phylogeny, and 1H NMR-based metabolomics into a polyphasic approach.
Results
Twenty-three strains were morphologically identified as Cladobotryum apiculatum, C. fungicola, C. mycophilum, C. varium, C. verticillatum, and Hypomyces rosellus (anamorph C. dendroides), whereas seven strains which produced red-pigmented metabolites presented an ambiguous taxonomic position at the species level. Molecular phylogenetics and metabolomics corroborated the morphological findings.
Conclusion
Thorough morphological study, ITS region-based phylogeny, and NMR-based metabolomics contribute complementarily to resolving the genus Cladobotryum systematics. The study demonstrated that combining multiple analytical approaches is necessary for accurate species identification and characterization within this genus.
- Published in:Journal of Fungi,
- Study Type:Laboratory Research Study,
- Source: 10.3390/jof8080877