Sexual Crossing, Chromosome-Level Genome Sequences, and Comparative Genomic Analyses for the Medicinal Mushroom Taiwanofungus camphoratus

Summary

This research provides the first detailed genetic blueprint of Taiwanofungus camphoratus, an important medicinal mushroom used in traditional Asian medicine. The scientists mapped out its complete genome and discovered why this mushroom is so rare in nature and difficult to grow commercially. The study revealed that the mushroom has lost many genes that other mushrooms use to break down plant material, which explains its unique growing requirements. Impacts on everyday life: • Helps develop better cultivation methods for this valuable medicinal mushroom • Could lead to more efficient production of natural medicines derived from the mushroom • May enable development of new therapeutic compounds for treating various diseases • Could reduce the cost of medicinal mushroom products through improved cultivation • Provides foundation for creating more resilient mushroom strains for commercial growing

Background

Taiwanofungus camphoratus mushrooms are used as complementary and alternative medicine for hangovers, cancer, hypertension, obesity, diabetes, and inflammation. While the mushroom has attracted biotechnological and pharmacological attention, proper classical genetic and genomic approaches have not been established for it.

Objective

To establish classical genetic and genomic approaches for T. camphoratus by isolating sexually competent monokaryons from commercial dikaryons and determining their chromosome-level genome sequences. The study aimed to enable better understanding of the biology, evolution, and secondary metabolite biosynthesis of this economically important medicinal mushroom.

Results

The study demonstrated that T. camphoratus has a tetrapolar mating system and that HC1 and SN1 represent two intraspecies isolates with karyotypic variation. Compared to other edible mushroom models, T. camphoratus showed significant contraction in gene family and individual gene numbers, particularly for plant, fungal, and bacterial cell-wall-degrading enzymes. This explains why these mushrooms are rare in natural environments, difficult to cultivate artificially, and susceptible to infections.

Conclusion

The research established the first proper genetic and genomic approaches for T. camphoratus study, including methods for precise genetic manipulation, improvements to mushroom fruiting, and synthetic biology applications for producing natural medicinal products. The findings revealed that T. camphoratus underwent retrogressive evolution, explaining many of its distinctive properties. The classical breeding method and comprehensive genomic data sets provide valuable resources for innovations in mushroom cultivation and biosynthesis of natural medicinal products.
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