Enhanced Enzymatic Hydrolysis of Cellulose from Substrate and Indole-3-Acetic Acid Content During the Fruiting Body Differentiation Stage by Sodium Acetate Addition

Summary

This research discovered that adding sodium acetate during mushroom cultivation can significantly improve the yield of straw mushrooms (Volvariella volvacea). The researchers found this works by helping break down the growing substrate more effectively and by increasing plant hormone levels that promote mushroom formation. This has practical implications for mushroom farmers and cultivation. Impacts on everyday life: – Could lead to more efficient mushroom production and lower costs for consumers – Provides a simple and cost-effective way for mushroom farmers to increase their yields – Demonstrates how basic compounds can be used to improve food production – Could help increase availability of nutritious mushrooms in tropical regions – Shows potential for improving cultivation of other mushroom species

Background

Volvariella volvacea is an important edible mushroom with high commercial, nutritional and medicinal value that is widely cultivated in tropical and subtropical regions. While supplementation effects on mushroom yield have been studied, the mechanisms behind yield improvements from sodium acetate addition were not well understood.

Objective

To investigate the effects of different concentrations and application timing of sodium acetate (NaAc) on V. volvacea yield and determine the underlying mechanisms related to enzymatic activity and hormone content during fruiting body development.

Results

The optimal application was 0.08% NaAc during substrate mixing, which increased yields by up to 89.16%. Maximum enzyme activity occurred during fruiting body development. NaAc significantly increased IAA content in early fruiting stages. Treatment with acetate salts increased yield by an average of 30.22%. The improvements were linked to enhanced enzymatic hydrolysis of cellulose/hemicellulose and increased IAA levels promoting primordium differentiation.

Conclusion

NaAc supplementation improves V. volvacea yield through two main mechanisms: 1) Increasing pH, C/N ratio and cellulolytic enzyme activity which enhances substrate degradation and nutrient availability, and 2) Increasing IAA content during early fruiting body development which promotes primordium differentiation and fruiting body formation.
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