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Optimization for the Production of a Polyketone 3S,4S-DMD from Panus lecomtei by Submerged Fermentation

Summary

This research developed an improved method for producing an antibacterial compound from an edible mushroom species. Instead of traditional solid growing methods, they used liquid fermentation with carefully controlled conditions to dramatically increase production efficiency. This advancement has important implications for developing natural medicines. Impacts on everyday life: – Could lead to more affordable and accessible natural antibiotics – Demonstrates how traditional medicinal mushrooms can be used in modern medicine – Shows potential for more sustainable pharmaceutical production methods – May help address antibiotic resistance through new natural compounds – Could increase availability of beneficial compounds from rare mushroom species

Background

3,4-Dihydroxy-2,2-dimethyl-chroman derivatives have diverse physiological properties. A polyketone (3S,4S)-3,4-Dihydroxy-6-methoxy-2,2-dimethylchromom (3S,4S-DMD) with antibacterial activity was previously isolated from solid culture of the rare edible fungus Panus lecomtei. However, the yield was very low and production period too long using traditional solid culture methods.

Objective

To develop and optimize an efficient submerged fermentation process for enhanced production of 3S,4S-DMD from P. lecomtei by studying key fermentation factors and conditions.

Results

A maximum 3S,4S-DMD yield of 196.3 mg/L was achieved in flask culture using optimized conditions of 25.78 g/L glucose, 1.67 g/L MgSO4·7H2O, 40°C and 197 r/min – a 1.3-fold increase compared to non-optimized conditions. In the 5-L fermenter, yield further increased to 261.6 mg/L. The 3S,4S-DMD productivity reached 7.26 and 8.07 mg/g per day in flask and fermenter respectively, representing a 121-fold increase over solid fermentation.

Conclusion

The study successfully developed an optimized submerged fermentation method for producing 3S,4S-DMD from P. lecomtei that dramatically improved yields compared to traditional solid culture. This presents a potential industrial method for efficient production of this bioactive compound.
  • Published in:Mycology,
  • Study Type:Laboratory Optimization Study,
  • Source: 10.1080/21501203.2022.2036842
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