Transcriptome Analysis Revealed the Mechanism of Inhibition of Saprophytic Growth of Sparassis latifolia by Excessive Oxalic Acid
- Author: mycolabadmin
- 2022-11-16
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Summary
This research investigated why the edible mushroom Sparassis latifolia grows slowly when cultivated on pine sawdust. Scientists discovered that excessive oxalic acid produced by the fungus actually inhibits its own growth by shutting down important metabolic processes. This finding helps explain why commercial cultivation of this valuable mushroom has been challenging.
Impacts on everyday life:
– Could lead to improved cultivation methods for this nutritious and medicinal mushroom
– May help reduce production costs and increase availability of this valuable food source
– Provides insights that could benefit cultivation of other medicinal mushrooms
– Could contribute to more efficient production of natural medicines derived from this fungus
– Demonstrates how understanding molecular processes can solve practical farming challenges
Background
Sparassis latifolia is a highly valued edible fungus and crucial medicinal food resource due to its rich active ingredients and pharmacological effects. When grown on pine-sawdust-dominated substrate, excessive oxalic acid secretion inhibits mycelial growth and severely restricts cultivation development. However, the mechanism linking oxalic acid to slow mycelial growth remains unclear.
Objective
To investigate the transcriptome-based response of S. latifolia induced by different oxalic acid concentrations and understand the mechanism behind growth inhibition.
Results
The study identified 9,206 differentially expressed genes across three comparison groups, with 4,587 genes down-regulated and 5,109 up-regulated. Excessive oxalic acid primarily down-regulated genes related to carbohydrate utilization pathways, energy metabolism, amino acid metabolism, protein synthesis, glycan biosynthesis, and signal transduction pathways. Genes encoding wood-degrading enzymes were predominantly down-regulated in mycelia treated with excessive oxalic acid.
Conclusion
The research revealed that excessive oxalic acid inhibits S. latifolia growth by down-regulating multiple metabolic pathways critical for mycelial development, including lignocellulose degradation, carbohydrate metabolism, and signal transduction processes. This inhibition of key metabolic pathways appears to be the primary cause of slow saprophytic growth and extended cultivation cycles in S. latifolia.
- Published in:Cells,
- Study Type:Transcriptome Analysis,
- Source: 10.3390/cells11223636