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Analysis of Biochemical and Genetic Variability of Pleurotus ostreatus Based on β-Glucans and CDDP Markers

Summary

This research analyzed different strains of oyster mushroom to identify those with the highest levels of beneficial compounds called β-glucans. The study found significant differences in β-glucan content between different parts of the mushroom and between different strains, with some containing more than twice the amount of others. This has important implications for everyday life: • Better selection of mushroom strains for producing health supplements and functional foods • More informed choices for consumers seeking maximum health benefits from mushrooms • Improved efficiency in commercial production of beneficial mushroom compounds • Potential for developing new mushroom-based health products • Enhanced understanding of how to maximize nutritional value in mushroom cultivation

Background

Oyster mushroom (Pleurotus ostreatus) is one of the most cultivated edible and medicinal mushrooms worldwide. Despite its frequent cultivation, limited information is available on strain variability regarding β-glucan content. β-glucans are heterogeneous polysaccharides with important prebiotic functions and health benefits, extracted from sources like cereals, mushrooms, seaweeds, and microorganisms.

Objective

To analyze the variability in β-glucan content across 60 strains of Pleurotus ostreatus, specifically examining concentrations in both caps and stipes. Additionally, to confirm strain authenticity through genome variability analysis using CDDP (Conserved DNA-derived polymorphism) markers.

Results

The stipe contained on average 33% more β-glucans than the cap. Minimum β-glucan content in stipes was 22% (strain 72) and maximum was 56% (strain 43). CDDP analysis revealed identical profiles between samples 45, 89, 95, and 96. The marker technique showed high polymorphism and effective discriminative power. Medium positive correlations were found between CDDP profiles and β-glucan content in strains with identical CDDP profiles.

Conclusion

Significant variability exists in β-glucan content between different P. ostreatus strains and between caps versus stipes. Strain 43 showed particular promise for commercial β-glucan production. CDDP markers proved effective for analyzing genetic polymorphism in P. ostreatus strains. The findings enable selection of high β-glucan producing strains for functional food and supplement production.
  • Published in:Journal of Fungi,
  • Study Type:Laboratory Research Study,
  • Source: 10.3390/jof8060563
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