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Immunostimulatory Effect of Postbiotics Prepared from Phellinus linteus Mycelial Submerged Culture via Activation of Spleen and Peyer’s Patch in C3H/HeN Mice

Summary

This research investigated how extracts from mushroom mycelium (the root-like structure of mushrooms) can boost the immune system. Scientists found that giving mice an extract from Phellinus linteus mushroom mycelium strengthened their immune response in multiple ways. This has important implications for human health: • Could lead to new natural supplements for improving immune system function • Provides a sustainable way to produce immune-boosting compounds without harvesting whole mushrooms • May help protect against infections and disease by enhancing the body’s natural defenses • Offers potential for developing new functional foods and supplements • Could be particularly beneficial for people with weakened immune systems

Background

Medicinal mushrooms have been traditionally used as valuable edible and medicinal food ingredients, with both Eastern and Western medical systems recognizing their therapeutic benefits. Phellinus linteus, known as ‘sangwhang’ in Korea, has been used as a medicinal mushroom for over 2000 years and possesses various therapeutic effects. While fruiting bodies have been well-studied, less research has focused on the potential benefits of mycelium produced through submerged cultivation.

Objective

To evaluate the immunostimulatory effects of postbiotics prepared from Phellinus linteus mycelial submerged culture (PLME) through investigation of spleen and intestinal immune system activation in C3H/HeN mice.

Results

PLME administration was well-tolerated and safe at all tested doses. The treatment significantly increased mitogenic activity of splenocytes and production of immunostimulatory cytokines (IFN-γ, TNF-α, IL-6). Serum IgG levels and intestinal IgA levels were elevated. PLME activated the intestinal immune system as evidenced by increased immune cell infiltration and enhanced Peyer’s patch-mediated bone marrow cell proliferation. Chemical analysis revealed PLME contained mainly neutral sugars (969.1 mg/g) with glucose as the primary monosaccharide unit (93.8%) and β-1,3:1,6-glucan content of 88.5 mg/g.

Conclusion

Oral administration of PLME demonstrates immunostimulatory activity by activating both spleen and intestinal immune tissues in normal C3H/HeN mice. The study suggests that mannose-containing heteropolysaccharides and β-1,3:1,6-glucans may be responsible for the immunostimulatory effects. This research provides foundational evidence for using PLME as a functional ingredient to promote immune activity.
  • Published in:Pharmaceuticals (Basel),
  • Study Type:Experimental Animal Study,
  • Source: 10.3390/ph15101215
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