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The Cold-Resistance Mechanism of a Mutagenic Volvariella volvacea Strain VH3 with Outstanding Traits Revealed by Transcriptome Profiling

Summary

This research investigated how a specially modified strain of edible straw mushroom can better survive cold temperatures compared to regular strains. The findings help address a major challenge in mushroom farming where cold storage often damages these valuable food crops. Impact on everyday life: – Improved storage and transportation of mushrooms leading to less food waste – Potential for longer shelf life of straw mushrooms in grocery stores – More reliable supply of nutritious mushrooms for consumers – Lower costs for mushroom producers and potentially lower prices for consumers – Foundation for developing other cold-resistant mushroom varieties

Background

The straw mushroom (Volvariella volvacea) is an important edible fungus popular for its delicious taste and health benefits, including cancer prevention, blood pressure reduction, and cholesterol lowering properties. However, it is highly sensitive to low temperatures, with mycelium growing slowly below 15°C and experiencing cryogenic autolysis below 4°C, causing significant economic losses during transportation and storage.

Objective

To investigate the molecular mechanism behind the increased cold resistance of a novel straw mushroom strain VH3 obtained through ultraviolet mutagenesis, compared to the ordinary cultivar V23.

Results

VH3 exhibited significantly higher cold resistance compared to V23, with lower electrolyte leakage and MDA contents, and higher SOD and CAT activities. Transcriptome sequencing revealed 111 differentially expressed genes between V23 and VH3 at 0h, and 117 DEGs at 4h of cold treatment. Six key genes were identified: VVO_00021 and VVO_00017 were up-regulated while VVO_00003, VVO_00004, VVO_00010, and VVO_00030 were down-regulated. These genes were annotated to pathways related to cold stress response. Additionally, GA3 content was decreased in VH3.

Conclusion

The increased cold resistance of VH3 strain appears to be caused by the expression changes of specific genes (VVO_00003, VVO_00004, VVO_00017, VVO_00021, and VVO_00030) and decreased GA3 content. This study provides new insights into the molecular mechanisms of cold resistance in straw mushroom.
  • Published in:BMC Microbiology,
  • Study Type:Experimental Research,
  • Source: 10.1186/s12866-021-02396-8
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