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Improved Chrysin Production by a Combination of Fermentation Factors and Elicitation from Chaetomium globosum

Summary

This research demonstrates an improved method for producing chrysin, a beneficial natural compound, using marine fungi. Instead of extracting chrysin from traditional sources like honey or plants, which is inefficient and unsustainable, the researchers developed a method using fungi that can produce much higher amounts of chrysin in controlled laboratory conditions. This has important implications for everyday life: • More sustainable and efficient production of natural health supplements • Potential for cheaper and more widely available natural medicines • Reduced environmental impact compared to traditional extraction methods • New ways to produce beneficial compounds without relying on plant sources • Advancement in natural drug production techniques that could be applied to other beneficial compounds

Background

Flavonoids are a heterogeneous group of secondary metabolites with exceptional health benefits. Chrysin, a natural dihydroxyflavone, has numerous bioactive properties including anticancer, antioxidative, antidiabetic, and anti-inflammatory effects. Traditional sources of chrysin involve extracting from honey and plants, which is non-scalable and unsustainable. Microbial production offers advantages of cost-effectiveness, easy scale-up, sustainability, and low waste emission.

Objective

To enhance the production of chrysin from the marine endophytic fungus Chaetomium globosum through optimization of fermentation parameters, feeding of key flavonoid pathway intermediates, and elicitation with biotic and abiotic factors. The study also aimed to assess the presence of flavonoid pathway intermediates in C. globosum extracts using LC-MS/MS.

Results

The combined effect of optimized parameters resulted in a 97-fold increase in chrysin yield, from 2.53 mg/g to 5.18 mg/g. The optimal conditions included M1D medium, 28 days incubation at 32°C, with supplementation of 5 μM phenylalanine, 1 mM cinnamic acid, 300 μg/mL yeast extract, 0.075% sodium alginate, 5 min UV exposure, 3.5% NaCl, and 0.05 mM CdCl2. Several key metabolites of the flavonoid biosynthesis pathway were detected in the fungal extracts.

Conclusion

The study demonstrated successful enhancement of chrysin production through bioprocess optimization strategies and established the significance of exogenous supplementation of precursors and elicitors. The work provides a template for enhanced production of secondary metabolites from marine endophytic fungi and underscores a sustainable route for producing desired compounds.
  • Published in:Microorganisms,
  • Study Type:Laboratory Research,
  • Source: 10.3390/microorganisms11040999
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