Microbiome Community Structure and Functional Gene Partitioning in Different Micro-Niches Within a Sporocarp-Forming Fungus
- Author: mycolabadmin
- 2021-03-30
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Summary
This research examined the microbial communities living within different parts of an edible mushroom called Thelephora ganbajun. The study revealed that different sections of the mushroom host distinct communities of bacteria with specific functions, much like different neighborhoods in a city have different types of businesses and services. This discovery has important implications for everyday life:
• Better understanding of how to potentially cultivate this valuable edible mushroom, which could improve food security and local economies
• Insights into how fungi contribute to forest health and ecosystem functioning
• Potential applications in sustainable agriculture and forestry practices
• Improved knowledge of natural processes that could inspire biotechnology innovations
• Better understanding of how microorganisms work together in nature, which could inform human health research
Background
Thelephora ganbajun is a wild edible mushroom highly valued in China. While microbiomes of some fungal sporocarps have been studied, their potential functional roles remain largely uncharacterized. T. ganbajun establishes ectomycorrhizal symbioses with various host trees and plays an important role in forest health and functioning.
Objective
To define the taxonomic and functional attributes within three micro-niches (compartments) of T. ganbajun using functional gene microarrays and amplicon sequencing. The study aimed to determine if microbial communities and their potentially active functions could be shaped by different compartments of the sporocarp-forming fungus.
Results
The diversity and composition of bacterial taxa and their functional genes differed significantly among the compartments. Among 31,117 functional genes detected, some were exclusively recorded in specific compartments: 1,334 genes involved in carbon and nitrogen fixation in the context; 524 genes influencing carbon and sulfite reduction in the hymenophore; and 255 genes involved in sulfur oxidation and polyphosphate degradation in the pileipellis. The context compartment showed the highest bacterial diversity and functional gene numbers.
Conclusion
The study revealed previously unknown microbiome and functional gene partitioning in sporome compartments of Basidiomycota, demonstrating higher genomic complexity than previously thought. The findings provide novel insights into how fungal tissue compartments drive microbiome composition and functional gene diversity, with implications for understanding ecological and biogeochemical functions of sporocarp-forming fungi in forest ecosystems.
- Published in:Frontiers in Microbiology,
- Study Type:Experimental Research,
- Source: 10.3389/fmicb.2021.629352