RNA-Seq Profiling Showed Divergent Carbohydrate-Active Enzymes (CAZymes) Expression Patterns in Lentinula edodes at Brown Film Formation Stage Under Blue Light Induction

Summary

This research examined how blue light affects the development of shiitake mushrooms, specifically during a crucial growth stage called brown film formation. The study found that blue light helps mushrooms form this brown coating more effectively and increases their production of beneficial compounds called polysaccharides. Impact on everyday life: – Improved methods for growing higher quality shiitake mushrooms commercially – Better understanding of how to control mushroom development using light – Potential for enhancing nutritional content of cultivated mushrooms – More efficient and productive mushroom farming techniques

Background

Lentinula edodes (shiitake mushroom) is one of the most important edible mushrooms worldwide. The L. edodes cultivation cycle includes a unique developing stage called brown film formation that directly affects the development of primordium and the quality of fruiting body. Brown film formation is induced by light, especially blue light.

Objective

To promote understanding of the role of blue light in brown film formation mechanisms of L. edodes by comparing transcriptomes of L. edodes grown under blue light and in dark conditions, and validating expression profiles using qRT-PCR.

Results

Blue light stimulated brown film formation and increased polysaccharide content in L. edodes. Among 730 differentially expressed genes (DEGs), 433 were up-regulated and 297 down-regulated. Most DEGs were in the oxidoreductase activity group. Pentose and glucuronic acid conversion and starch/sucrose metabolism were the most important pathways. 79 DEGs were identified as genes encoding carbohydrate-active enzymes (CAZymes), with 51 being up-regulated.

Conclusion

Blue light promotes brown film formation and increases polysaccharide content in L. edodes by enhancing enzyme activities. CAZymes play important roles in providing nutrition during brown film formation through substrate degradation. The results provide insights into genes involved in L. edodes developmental control.
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