Effects of Medium Composition and Genetic Background on Agrobacterium-mediated Transformation Efficiency of Lentinula edodes
- Author: mycolabadmin
- 2019-06-19
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Summary
This research developed an improved method for genetically modifying shiitake mushrooms in the laboratory. The study found that the growth medium and genetic makeup of the mushroom strains significantly affect how well they can be modified. This advancement helps scientists better understand and potentially improve mushroom characteristics.
Impacts on everyday life:
• Could lead to improved shiitake mushroom varieties with better growth or nutritional properties
• May help develop mushrooms with enhanced medicinal compounds
• Could contribute to more efficient and sustainable mushroom cultivation methods
• May lead to better understanding of how to improve other edible and medicinal mushrooms
• Could help develop new biotechnology applications using mushrooms
Background
Filamentous fungi, particularly basidiomycetes like Lentinula edodes (shiitake mushroom), play important roles in agriculture, industry, and medicine. While genome sequences of many filamentous fungi are now available, functional genomics research requires efficient genetic transformation methods. Existing transformation methods for L. edodes have shown insufficient efficiency to support large-scale functional genomics research.
Objective
To establish and optimize an efficient Agrobacterium-mediated transformation method for both monokaryon and dikaryon strains of L. edodes by evaluating the effects of medium composition and genetic background on transformation efficiency.
Results
The highest transformation efficiency achieved was 32.96% germination frequency and 85.12% positive frequency in hygromycin-resistant transformants. Medium composition significantly affected transformation efficiency, with different optimal media for monokaryon versus dikaryon strains. The A. tumefaciens strain EHA105 showed better performance than AGL1. Genetic background of recipient strains strongly influenced transformation success, with strain YS3334 showing the highest efficiency.
Conclusion
The study successfully established an efficient Agrobacterium-mediated transformation protocol for both monokaryon and dikaryon strains of L. edodes. Medium composition for recipient incubation and genetic background significantly affected transformation efficiency. This optimized method provides a practical approach for functional genomics research in L. edodes and potentially other basidiomycetes.
- Published in:Genes (Basel),
- Study Type:Laboratory Research,
- Source: 10.3390/genes10060467