MYCOLab Ai Logo - gold and army green

GSNOR Regulates Ganoderic Acid Content in Ganoderma lucidum Under Heat Stress Through S-nitrosylation of Catalase

Summary

This research reveals how a specific enzyme (GSNOR) controls the production of beneficial compounds in the medicinal mushroom Ganoderma lucidum when exposed to heat stress. The study shows that GSNOR works by modifying another enzyme (catalase) through a process called S-nitrosylation, which helps the mushroom manage stress and regulate its production of medicinal compounds. Impacts on everyday life: – Helps improve cultivation methods for medicinal mushrooms – Advances understanding of how organisms respond to environmental stress – Could lead to better methods for producing natural medicinal compounds – Provides insights for developing stress-resistant crops – Contributes to understanding natural antioxidant mechanisms

Background

S-nitrosoglutathione (GSNO) reductase (GSNOR) is a master regulator that balances NO signaling and protein S-nitrosylation, involved in various developmental processes and stress responses. However, the specific proteins and sites that can be S-nitrosylated, especially in microorganisms, and their physiological functions remain unclear.

Objective

To investigate how GSNOR regulates ganoderic acid content via protein S-nitrosylation under heat stress conditions in Ganoderma lucidum, and to identify the targets for S-nitrosylation and understand the regulation of protein function by S-nitrosylation in microorganisms.

Results

The ganoderic acid content in GSNOR-silenced strains was significantly lower (by 25%) than wild type under heat stress. Silencing GSNOR resulted in an 80% increase in catalase activity, which decreased GA accumulation via inhibition of ROS signaling. CAT was found to be S-nitrosylated both in vitro and in vivo. Three specific cysteine residues (Cys401, Cys642 and Cys653) in CAT were identified as S-nitrosylation sites, with Cys401 playing a pivotal role in CAT activity.

Conclusion

GSNOR regulates ganoderic acid content by controlling ROS levels through S-nitrosylation of catalase under heat stress conditions. The study identified specific S-nitrosylation sites on catalase and demonstrated their functional importance, particularly Cys401. This establishes a molecular framework for understanding how GSNOR functions during ROS homeostasis control.
  • Published in:Communications Biology,
  • Study Type:Experimental Research,
  • Source: 10.1038/s42003-021-02988-0
Scroll to Top