MYCOLab Ai Logo - gold and army green

Transcriptome Analysis of the Response of Hypomyces chrysospermus to Cadmium Stress

Summary

This research examined how a fungus called Hypomyces chrysospermus responds to and survives toxic cadmium exposure at the genetic level. The study revealed multiple defense mechanisms the fungus uses to protect itself from cadmium toxicity, including special transport proteins, antioxidant systems, and stress response genes. Impacts on everyday life: – Provides new ways to clean up toxic heavy metal pollution using fungi – Helps develop more effective environmental remediation strategies – Advances our understanding of how organisms adapt to toxic substances – Could lead to improved methods for treating contaminated soil and water – May help create safer food by reducing heavy metal contamination

Background

Hypomyces chrysospermus is a fungal parasite that grows on Boletus species. A strain isolated from B. griseus was previously found to have strong ability to tolerate and absorb cadmium (Cd). However, the molecular mechanisms underlying H. chrysospermus’s resistance to Cd stress have not been fully investigated.

Objective

This study aimed to assess the effect of Cd stress on the global transcriptional regulation of H. chrysospermus and identify the molecular mechanisms involved in its Cd tolerance.

Results

A total of 1,839 differentially expressed genes were identified under 120 mg/l Cd stress. GO enrichment analysis revealed large amounts of DEGs were associated with cell membrane components, oxidoreductase activity, and transport activity. KEGG analysis showed DEGs were mainly involved in translation, amino acid metabolism, transport and catabolism, carbohydrate metabolism, and folding/sorting and degradation pathways. The expression of genes encoding transporter proteins, antioxidant enzymes, nonenzymatic antioxidant proteins, detoxification enzymes, and transcription factors was associated with the Cd stress response.

Conclusion

The study revealed multiple molecular mechanisms involved in H. chrysospermus’s response to Cd stress, including metal transport systems, antioxidant defenses, and transcriptional regulation. These findings provide insights into fungal Cd tolerance mechanisms and may facilitate development of improved fungal bioremediation strategies.
  • Published in:Frontiers in Microbiology,
  • Study Type:Transcriptome Analysis,
  • Source: 10.3389/fmicb.2022.990693
Scroll to Top