Lentinula edodes Mycelium as Effective Agent for Piroxicam Mycoremediation
- Author: mycolabadmin
- 2019-02-21
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Summary
This research demonstrates how mushroom cultures can be used to clean up pharmaceutical pollution from the environment. Scientists found that the mycelium (root system) of the edible mushroom Lentinula edodes can effectively break down piroxicam, a common anti-inflammatory drug that often ends up as an environmental contaminant. The implications for everyday life include:
• Provides a natural, environmentally-friendly way to remove drug pollution from water systems
• Offers a cost-effective alternative to conventional wastewater treatment methods
• Demonstrates potential for using edible mushrooms in environmental cleanup
• Could help reduce the impact of pharmaceutical waste on aquatic ecosystems
• Shows promise for developing new green technologies for water purification
Background
Environmental pollution with inorganic and organic substances, including unmetabolized pharmaceutical drugs, is a major global concern. White rot fungi like Lentinula edodes show promise for biodegradation of xenobiotics due to their ability to produce oxidizing enzymes that can break down these compounds. L. edodes is an edible mushroom species known for various therapeutic effects including anti-cancer, hypocholesterolemic, hypotensive, hypoglycemic and antioxidant properties.
Objective
To investigate if in vitro cultures of Lentinula edodes can be effectively used for bioremediation of the non-steroidal anti-inflammatory drug piroxicam, and to analyze the biodegradation pathway using UPLC/MS/MS methods.
Results
The degradation process primarily affected the linker between the thiazine and piperidine ring, leading to oxidation and cleavage. Additional oxidation of the benzothiazine moiety resulted in hydroxylation and oxidation of the phenyl ring, as well as oxidation of the thiazine ring leading to partial or complete removal of the sulfonamide moiety. The final degradation product appeared to be 2-hydroxybenozquinone. The concentration of piroxicam did not correlate with degradation effectiveness, as the level of degradation remained consistent across all experimental series.
Conclusion
The mycelium of L. edodes demonstrated effective ability to degrade piroxicam in a short time period, suggesting its potential use as an alternative method for remediating piroxicam contamination. The reproducible culture conditions allowed for monitoring of accumulation and biodegradation, making this approach promising for environmental remediation applications.
- Published in:Frontiers in Microbiology,
- Study Type:Laboratory Research,
- Source: 10.3389/fmicb.2019.00313