Bioprocessing of Agricultural Residues as Substrates and Optimal Conditions for Phytase Production of Chestnut Mushroom, Pholiota adiposa, in Solid State Fermentation
- Author: mycolabadmin
- 2020-12-21
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Summary
This research explored using agricultural waste materials to produce an important enzyme called phytase using mushrooms. The enzyme helps animals better absorb phosphorus from their food. The study found that the chestnut mushroom (Pholiota adiposa) could effectively produce this enzyme when grown on water hyacinth, a common aquatic weed. The researchers optimized the production conditions to get maximum enzyme yields.
Impacts on everyday life:
– Provides a way to convert agricultural waste into valuable products
– Could lead to more sustainable and cost-effective animal feed supplements
– Helps reduce environmental pollution from agricultural waste
– Demonstrates potential for using invasive plants (water hyacinth) productively
– Could lower costs of enzyme production for various industrial applications
Background
Phytase is an enzyme that breaks down phytates to release phosphorus in an available form. This enzyme plays an important role in animals, especially monogastric animals, by improving phytate digestion and phosphorus absorption needed for optimal growth and health. Agricultural residues are lignocellulosic materials that can potentially be used as substrates for microbial enzyme production through solid-state fermentation.
Objective
To screen for effective phytase-producing mushroom strains and select agricultural residues for phytase production under solid-state fermentation. Additionally, to determine the optimal conditions for phytase production using statistical approaches and characterize the enzyme’s properties.
Results
Five mushroom species showed positive phytase production. P. adiposa produced the highest phytase yield (17.02 U/gds) using water hyacinth as substrate. Statistical optimization increased enzyme production by 3.15-fold (53.66 U/gds) under optimal conditions: 85% moisture content, pH 6.5, 30°C for 7 days. The precipitated enzyme showed highest activity at 42°C and pH 5.0, with Fe2+ enhancing activity. The enzyme was most stable at pH 8.0 and 4°C.
Conclusion
P. adiposa effectively produces phytase using water hyacinth as substrate under solid-state fermentation. Statistical optimization significantly improved enzyme yields. The enzyme shows good stability and activity under specific conditions, suggesting potential for industrial applications in food additives, animal feeds, and medical uses.
- Published in:Journal of Fungi,
- Study Type:Laboratory Research,
- Source: 10.3390/jof6040384