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Expression Patterns of Two PAL Genes of Pleurotus ostreatus Across Developmental Stages and Under Heat Stress

Summary

This research investigated two important genes in oyster mushrooms that affect how the mushrooms develop and respond to heat stress. The study provides insights into how mushrooms can be made more resilient to temperature changes during cultivation. Impacts on everyday life: – Could lead to more heat-resistant mushroom varieties for farmers – May help improve mushroom cultivation in warmer climates – Could contribute to developing more efficient mushroom production methods – May help reduce crop losses due to heat stress – Could lead to better understanding of how to grow mushrooms year-round

Background

Phenylalanine ammonia-lyase (PAL) is the first key enzyme in the phenylpropanoid pathway, catalyzing the conversion of L-phenylalanine to trans-cinnamic acid. While PAL has been widely studied in plants for its role in growth, development and defense systems, its biological functions during development and abiotic stress response in Pleurotus ostreatus have not been reported.

Objective

To clone and characterize two PAL genes (pal1 and pal2) from P. ostreatus CCMSSC 00389 and investigate their roles in mushroom development and heat stress response through overexpression and RNA interference experiments.

Results

The pal1 (2232 bp) and pal2 (2244 bp) genes were successfully cloned and characterized. Both genes showed similar expression patterns during development, with higher expression in reproductive versus vegetative growth stages. Interference of pal1 and pal2 delayed primordia formation. Under heat stress, RNAi-pal1 strains showed enhanced mycelial tolerance to high temperature, while RNAi-pal2 strains demonstrated enhanced resistance to H2O2.

Conclusion

The two PAL genes appear to play similar roles in P. ostreatus fruiting body development but may alleviate stress through different regulatory pathways under heat stress conditions. The research provides new insights into PAL gene function in mushroom development and stress response.
  • Published in:BMC Microbiology,
  • Study Type:Experimental Research,
  • Source: 10.1186/s12866-019-1594-4
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