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The Transformation and Protein Expression of the Edible Mushroom Stropharia rugosoannulata Protoplasts by Agrobacterium-tumefaciens-Mediated Transformation

Summary

Scientists developed a genetic engineering technique to modify king stropharia (a cultivated edible mushroom) by inserting foreign genes into its cells. This breakthrough allows researchers to study how the mushroom grows and produces beneficial compounds. The technique uses a bacterium called Agrobacterium tumefaciens to naturally deliver genes into mushroom cells, similar to how it infects plants. This advancement could lead to improved cultivation practices and enhanced nutritional or medicinal properties.

Background

Stropharia rugosoannulata is a cultivated edible mushroom with significant nutritional and enzymatic value, but lacks genetic tools for studying its molecular mechanisms. The absence of functional genetic transformation systems has severely limited research on functional genomics and precision breeding in this species.

Objective

This study aimed to establish an Agrobacterium-tumefaciens-mediated transformation (ATMT) system for S. rugosoannulata protoplasts to enable exogenous and endogenous gene expression. The researchers sought to develop genetic tools including selectable marker genes and reporter genes for functional genomic research.

Results

The ATMT system successfully achieved stable expression of exogenous genes (mCherry, hph, GUS) and endogenous mutant SDI gene in S. rugosoannulata. Transformation efficiency was approximately 1.17‰ with hph and 1.56‰ with mSDI markers. Fluorescence microscopy confirmed uniform distribution of mCherry protein, and GUS activity was successfully detected through X-gluc staining.

Conclusion

This study establishes the feasibility of ATMT for S. rugosoannulata genetic transformation and provides essential genetic tools for future functional genomics and precision breeding research. The developed system enables stable expression of selectable markers and reporter genes critical for investigating gene function and improving cultivation practices.
  • Published in:Journal of Fungi,
  • Study Type:Experimental Research,
  • Source: PMID: 41003220, DOI: 10.3390/jof11090674
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