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The VlMYB149-VlHIPP30 Regulatory Module Enhances Grapevine Resistance to Botrytis cinerea by Activating the Antioxidant System and Copper Metabolism

Summary

Scientists discovered that two grape proteins working together help grapes resist a common fungal disease called grey mould. When grapes are infected, one protein (VlMYB149) activates a second protein (VlHIPP30) that helps the plant accumulate copper and boost its natural antioxidant defenses. This discovery could help farmers grow disease-resistant grape varieties without relying on chemical fungicides.

Background

Grapevine is highly susceptible to grey mould caused by Botrytis cinerea, leading to significant yield and quality decline. Current chemical control methods have environmental and resistance limitations. Copper and antioxidant systems play important roles in plant disease resistance mechanisms.

Objective

To identify and characterize a regulatory module involving VlMYB149 transcription factor and VlHIPP30 copper transport protein that enhances grapevine resistance to Botrytis cinerea through activation of antioxidant enzymes and copper metabolism.

Results

VlMYB149 overexpression significantly enhanced resistance to B. cinerea in both grapevine and Arabidopsis, with reduced lesion size and fungal biomass. VlMYB149 directly activated VlHIPP30 expression by binding to MYB binding sites in its promoter. Both genes increased copper content and antioxidant enzyme activities (CAT, POD, SOD) while reducing ROS accumulation.

Conclusion

The VlMYB149-VlHIPP30 regulatory module enhances grapevine resistance to grey mould through synergistic activation of copper metabolism and the antioxidant system. This study provides genetic resources for breeding disease-resistant grapevines and advances understanding of plant immunity mechanisms through metal homeostasis and ROS scavenging.
  • Published in:Molecular Plant Pathology,
  • Study Type:Experimental Research,
  • Source: 41521392
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