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Analysis of Gene Regulatory Network and Transcription Factors in Different Tissues of the Stropharia rugosoannulata Fruiting Body

Summary

Researchers analyzed the gene activity patterns across different parts of wine cap mushrooms (Stropharia rugosoannulata) to understand how the fruiting body develops. By examining gene expression in six different tissue types, they identified which genes are active in each tissue and what biological processes they control. This foundational knowledge can help improve mushroom cultivation techniques and production efficiency.

Background

Stropharia rugosoannulata is a nutritionally rich mushroom with expanding cultivation due to its simplicity and diversity. However, the molecular mechanisms governing fruiting body development, which constitutes the edible portion, remain incompletely understood.

Objective

To elucidate the developmental mechanisms of S. rugosoannulata fruiting bodies by analyzing gene expression patterns, gene regulatory networks, and transcription factors across different tissue types including pileipellis, pileus, gill, veil, stipe, and trama.

Results

Sixteen co-expression gene modules were identified with six showing tissue-specific expression patterns. Tissue-specific pathways included amino acid metabolism in pileus, sugar metabolism in stipe, tryptophan metabolism and wax production in pileipellis, DNA replication pathways in gill, amino sugar metabolism in veil, and nitrogen metabolism in trama. Fifteen tissue-specific transcription factors from five gene families were identified, with promoter analysis revealing roles for light response, methyl jasmonate, oxygen, and temperature signaling.

Conclusion

This study provides comprehensive molecular evidence for fruiting body development mechanisms in S. rugosoannulata through transcriptome analysis and gene regulatory network characterization. The identified tissue-specific genes, transcription factors, and metabolic pathways offer insights for enhancement of cultivation techniques.
  • Published in:Journal of Fungi,
  • Study Type:Transcriptomic Analysis Study,
  • Source: PMC11856722; PMID: 39997417
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