MYCOLab Ai Logo - gold and army green

Mechanism Underlying Ganoderma lucidum Polysaccharide Biosynthesis Regulation by the β-1,3-Glucosyltransferase Gene gl20535

Summary

Researchers studied a gene called gl20535 in the medicinal mushroom Ganoderma lucidum that controls how the fungus makes beneficial polysaccharides. When they increased this gene’s activity, the mushroom produced significantly more polysaccharides with improved composition. The gene works by controlling sugar pathways and related enzyme production, and the mushroom compensates when this gene is reduced. These findings could help improve the production of medicinal mushroom products for food and health applications.

Background

Ganoderma lucidum polysaccharides (GLPs) are natural compounds with broad spectrum biological activities including immunoregulatory and anti-inflammatory properties. β-1,3-glucosyltransferase (GL20535) plays an important role in polysaccharide synthesis by catalyzing the transfer of UDP-glucose to extend sugar chains. However, the underlying regulatory mechanism of GL20535 in polysaccharide biosynthesis remains unclear.

Objective

To elucidate the regulatory mechanism of GL20535 in polysaccharide synthesis in G. lucidum by constructing and analyzing overexpression and silencing strains. The study aimed to determine how GL20535 influences intracellular polysaccharide (IPS), extracellular polysaccharide (EPS), and β-1,3-glucan contents.

Results

Overexpression of gl20535 resulted in maximum increases of 18.08% in IPS, 79.04% in EPS, and 18.01% in β-1,3-glucan contents. Silencing gl20535 caused maximum reductions of 16.97% in IPS, 30.20% in EPS, and 23.56% in β-1,3-glucan contents. GL20535 promoted UDP-glucose synthesis and upregulated glycoside hydrolase genes in overexpression strains, with compensatory upregulation of isozyme gene gl24465 in silenced strains.

Conclusion

β-1,3-glucosyltransferase (GL20535) plays an important regulatory role in polysaccharide synthesis by influencing UDP-glucose and GDP-mannose synthesis pathways, regulating glycoside hydrolase genes for EPS accumulation, and maintaining compensatory relationships with isozyme gl24465. These findings deepen understanding of GL20535 function in the polysaccharide biosynthesis network of edible and medicinal fungi.
  • Published in:Journal of Fungi,
  • Study Type:Experimental Study,
  • Source: 40985438
Scroll to Top