MYCOLab Ai Logo - gold and army green

Biological Characteristics, Domesticated Cultivation Protocol, Antioxidant Activity, and Protective Effects against Cellular Oxidative Stress of an Underutilized Medicinal Mushroom: Fomitopsis palustris

Summary

Researchers successfully grew and studied Fomitopsis palustris, a medicinal mushroom, in laboratory conditions. They discovered that the mushroom contains beneficial compounds that protect cells from damage caused by harmful free radicals. The polysaccharides extracted from the mushroom boosted the cells’ natural defense systems and reduced oxidative stress markers, suggesting potential health benefits for treating age-related and degenerative diseases.

Background

Fomitopsis palustris is a brown-rot fungus belonging to the genus Fomitopsis, which includes important medicinal mushroom species. While some Fomitopsis species have been extensively studied for medicinal properties, F. palustris has been primarily investigated for enzyme activity and brown rot characteristics. Recent studies suggest cytotoxic potential against tumor cell lines.

Objective

This study aimed to identify and characterize F. palustris collected from Liaoning Province, establish optimal cultivation conditions through single-factor and orthogonal testing, develop domesticated cultivation protocols, and evaluate the antioxidant activity and protective effects of crude polysaccharides against cellular oxidative stress.

Results

Optimal mycelium culture conditions were glucose, yeast extract powder, pH 6.0, and 35°C, with temperature being the most significant factor. Cultivated fruiting bodies matured in approximately 60 days. Crude polysaccharides demonstrated significant DPPH and hydroxyl radical scavenging abilities, with EC50 values of 0.81 and 1.47 mg/mL respectively. FPPs effectively mitigated H2O2-induced oxidative stress by enhancing SOD and CAT activities and reducing ROS and MDA levels in RAW264.7 cells.

Conclusion

This study provides preliminary evidence of the medicinal and economic value of F. palustris through successful domestication and demonstration of antioxidant and cellular protective properties. The optimized cultivation protocol and characterization of polysaccharide bioactivity provide a foundation for future industrial development and therapeutic applications of this underutilized medicinal mushroom species.
  • Published in:Journal of Fungi,
  • Study Type:Experimental Research,
  • Source: 10.3390/jof10060380
Scroll to Top