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Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia

Summary

Researchers developed a new method to produce medicinal polysaccharides from a Cordyceps fungus using tiny talc particles to improve fermentation. By adding the right amount and size of talc particles, they were able to produce significantly more polysaccharides with beneficial health properties. The method works well in large-scale bioreactors and could be used to produce these valuable medicinal compounds more efficiently.

Background

Cordyceps polysaccharides are important bioactive compounds with multiple physiological functions. However, natural Cordyceps has become endangered due to overharvesting. Submerged fermentation using Paraisaria dubia, the anamorph of Ophiocordyceps gracilis, can produce polysaccharides, but mycelial pellet morphology limits nutrient delivery and polysaccharide yield.

Objective

To develop an enhanced fermentation strategy using talc microparticles as a morphological inducer to improve polysaccharide production by Paraisaria dubia and elucidate the molecular mechanisms underlying this enhancement.

Results

Optimal conditions of 2000 mesh talc at 15 g/L resulted in intracellular polysaccharide yield of 69.16 ± 5.4 mg/g and exopolysaccharide yield of 478.20 ± 16.3 mg/L in shake flasks. In 5-L bioreactor, yields reached 83.23 ± 1.4 mg/mL and 518.50 ± 4.1 mg/L respectively. Transcriptomics revealed ABC transporter-dependent pathway as the primary mechanism for polysaccharide synthesis.

Conclusion

Talc microparticle-enhanced fermentation successfully improved cordyceps polysaccharide production through morphological regulation and enhanced nutrient transfer. The strategy demonstrated robustness in bioreactor scale-up with maintained biological activity and shows potential for industrial application to similar macrofungi.
  • Published in:Microbial Cell Factories,
  • Study Type:Experimental Study,
  • Source: PMID: 35090444, DOI: 10.1186/s12934-021-01733-w
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